Conservation

Husband and Wife Team Have Big Ideas for Big Cat Conservation

Not everyone can say that they passed on an offer to study at Oxford University. Mason doctoral students Trishna Dutta and Sandeep Sharma did just that in 2006, when the husband and wife pair received admission to pursue their graduate degrees in conservation. They chose Mason instead and are now close to finishing their doctorates through the environmental science and public policy program in the College of Science.

Why Mason? Among the factors are the school’s proximity to Washington, D.C., home to public policy’s movers and shakers, and its special relationship with the Smithsonian Conservation Biology Institute (SCBI) and the National Zoo, where they both are doctoral fellows.

Trishna Dutta collects field samples in India.

Dutta and Sharma’s research deals with one of the most pressing issues in conservation policy today: how to ensure the preservation of large cat species in India, the world’s second-most populous nation. Specifically, they’re studying whether forest corridors that connect five major tiger reserves in the central Indian states of Madhya Pradesh and Maharashtra are functioning for two species, the Indian leopard (Panthera pardus fusca) and the Indian tiger (Panthera tigris tigris).

To determine whether genes are flowing within tiger and leopard populations, Dutta and Sharma are using noninvasive genetic sampling, a technique they learned at SCBI, to extract an animal’s genetic material without trapping and handling it.The samples are not blood or tissue, but scat, fecal matter containing sloughed-off cells from the intestinal lining, from which DNA can be extracted.

The downside to this approach is that DNA tends to be “highly degraded,” says Sharma.

Trishna Dutta and Sandeep Sharma

“You have small pieces of DNA that are not really good,” explains Dutta, “so the whole emphasis on the method is to try to get as much information as you can while maintaining a strict quality control.”

Since returning from the field in 2010, the couple has spent countless hours in the lab working on the hundreds of scat samples they collected over two years. While leery of drawing firm conclusions yet, they’ve identified 217 individual leopards and 273 individual tigers from their samples, and preliminary results suggest that corridors are playing an important role in maintaining genetic diversity in these two species.

Ultimately, they say, sound conservation policy must be rooted in evidence-based science; they are hopeful that their work will help policy makers in India make better decisions regarding animal habitats.

“With this large sample size,” says Sharma, “we’ll have some conclusive results that you can transform into policy or management recommendations.”

Sandeep Sharma measures claw marks left on trees.

Partners in both work and life, Dutta and Sharma spend a lot of time together. They met in 2001 and worked on a research project on snow leopards and wolves in the trans-Himalayan region in northern India in 2004. The harsh environment made for a challenging experience, one that seemed to seal their bond.

“Once we pulled through that, we thought, if we can deal with this together, then I think we can deal with anything,” says Dutta. “Working as a couple makes field work enjoyable and navigating all the hurdles simpler. Whether it’s sampling in the field or writing papers, it’s a huge support to know that you have each other to fall back on.”

This article appeared in the spring 2012 issue of “Periodic Elements,” the College of Science newsletter.

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